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About High performance liquid chromatography (HPLC)
HPLC refers to an analytical method that separates the components contained in the sample in a column, detects it with a detector, and displays it as a chromatogram.
In HPLC, a device as shown in the figure below is used, and the pump transports the mobile phase (such as an organic solvent), and the sample injected through the injector enters the column, The separated components are detected by ultraviolet - visible absorption spectrophotometer, etc. The concentration can be known by comparison with the standard of known concentration.
It is used in a wide range of fields such as analysis of biological components, analysis of food ingredients, analysis of contaminants in the environment, analysis of impurities in pharmaceuticals, and confirmation of product processes.
In HPLC, a device as shown in the figure below is used, and the pump transports the mobile phase (such as an organic solvent), and the sample injected through the injector enters the column, The separated components are detected by ultraviolet - visible absorption spectrophotometer, etc. The concentration can be known by comparison with the standard of known concentration.
It is used in a wide range of fields such as analysis of biological components, analysis of food ingredients, analysis of contaminants in the environment, analysis of impurities in pharmaceuticals, and confirmation of product processes.